pVgRXR vector (V012768)

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LOCUS       V012768                 8803 bp    DNA     circular SYN 18-SEP-2021
DEFINITION  Exported.
ACCESSION   V012768
VERSION     V012768
KEYWORDS    pVgRXR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8803)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..359
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        789..836
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             856..1227
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    1294..1428
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1592..2180)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     enhancer        2265..2568
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2569..2772
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2907..2925
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3038..3271
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     polyA_signal    5535..5583
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6352..6613
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             6637..8022
                     /gene="RXRA"
                     /label="Retinoic acid receptor RXR-alpha"
                     /note="Retinoic acid receptor RXR-alpha from Homo sapiens.
                     Accession#: P19793"
     promoter        complement(8064..8082)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    8108..8332
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8378..8803
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"