Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012763 | pX260 | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA. Also known as pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
- Vector Name:
- pX260
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10203 bp
- Type:
- CRISPR Plasmids
- Replication origin:
- ori
- Selection Marker:
- Puromycin
- Copy Number:
- High copy number
- Promoter:
- mPGK
pX260 vector Vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pX260 vector Sequence
LOCUS pX260. 10203 bp DNA circular SYN 11-SEP-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pX260
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10203)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 10203)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10203
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 157..442
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
repeat_region 493..528
/label=DR
/note="DR"
/note="direct repeat for the Streptococcus pyogenes
CRISPR/Cas system"
repeat_region 545..580
/label=DR
/note="direct repeat for the Streptococcus pyogenes
CRISPR/Cas system"
promoter complement(591..831)
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
rep_origin complement(893..1481)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1655..2512)
/label=AmpR
/note="beta-lactamase"
promoter complement(2513..2617)
/label=AmpR promoter
rep_origin complement(2899..3354)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(3630..4226)
/label=PuroR
/note="puromycin N-acetyltransferase"
promoter complement(4266..4765)
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
protein_bind complement(4826..4859)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_RNA complement(5030..5108)
/label=tracrRNA
/note="trans-activating CRISPR RNA for the Streptococcus
pyogenes CRISPR/Cas9 system"
promoter complement(5119..5333)
/label=H1 promoter
/note="human H1 RNA promoter"
polyA_signal complement(5347..5554)
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
CDS complement(5588..5635)
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=bipartite nuclear localization signal from
nucl
/note="nucleoplasmin NLS"
/translation="KRPAATKKAGQAKKKK"
CDS complement(5636..9736)
/label=Cas9
/note="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
CDS complement(9761..9781)
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=nuclear localization signal of SV40 large T
ant
/note="SV40 NLS"
/translation="PKKKRKV"
CDS complement(9788..9853)
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=three tandem FLAG
/note="3xFLAG"
/translation="DYKDHDGDYKDHDIDYKDDDDK"
intron complement(9874..10101)
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"