Basic Vector Information
- Vector Name:
- PX551
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7400 bp
- Type:
- AAV plasmids
- Replication origin:
- ori
- Copy Number:
- High copy number
- Promoter:
- Mecp2
PX551 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
PX551 vector Sequence
LOCUS PX551. 7400 bp DNA circular SYN 11-SEP-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS PX551. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7400) TITLE Direct Submission REFERENCE 2 (bases 1 to 7400) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..7400 /mol_type="other DNA" /organism="synthetic DNA construct" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" promoter 160..388 /label=Mecp2 promoter /note="promoter for selective expression in neurons (Adachi et al., 2005; Gray et al., 2011)" CDS 404..430 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /note="HA" /translation="YPYDVPDYA" CDS 434..454 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=nuclear localization signal of SV40 large T ant /note="SV40 NLS" /translation="PKKKRKV" CDS 464..4564 /label=Cas9 /note="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" CDS 4568..4588 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=nuclear localization signal of SV40 large T ant /note="SV40 NLS" /translation="PKKKRKV" polyA_signal 4607..4655 /label=poly(A) signal /note="synthetic polyadenylation signal" repeat_region 4663..4803 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 4878..5333 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 5615..5719 /label=AmpR promoter CDS 5720..6577 /label=AmpR /note="beta-lactamase" rep_origin 6751..7339 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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