Basic Vector Information
N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).
- Vector Name:
- PX853
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6295 bp
- Type:
- CRISPR/Cas
- Replication origin:
- ori
- Copy Number:
- High copy number
- Promoter:
- CBh
PX853 vector Map
PX853 vector Sequence
LOCUS PX853. 6295 bp DNA circular SYN 11-SEP-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS PX853
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6295)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6295)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6295
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..241
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 268..343
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 440..725
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 727..1004
/label=chicken beta-actin promoter
intron 1005..1233
/note="hybrid intron"
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
CDS 1275..2993
/label=Cas9(N)
/note="N-terminal portion of Streptococcus pyogenes Cas9
(Zetsche et al., 2015)"
CDS 3039..3308
/label=FRB
/note="FKBP-rapamycin binding domain of human FRAP"
polyA_signal 3342..3549
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 3558..3698
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3773..4228
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4510..4614
/label=AmpR promoter
CDS 4615..5472
/label=AmpR
/note="beta-lactamase"
rep_origin 5646..6234
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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