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pCAG-Cre vector (V012688)

Basic Vector Information

Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

Vector Name:
pCAG-Cre
Antibiotic Resistance:
Ampicillin
Length:
5871 bp
Type:
CRE/LOXP
Replication origin:
ori
Source/Author:
Dr. Connie Cepko's lab
Copy Number:
High copy number
Promoter:
chicken β-actin
Expression Method:
Transient

pCAG-Cre vector Vector Map

pCAG-Cre5871 bp60012001800240030003600420048005400CMV enhancerchicken beta-actin promoterchimeric intronCreMycbeta-globin poly(A) signalM13 revlac operatorlac promoterCAP binding siteSV40 promoterSV40 poly(A) signaloriAmpRAmpR promoter

pCAG-Cre vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pCAG-Cre.        5871 bp DNA     circular SYN 06-APR-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pCAG-Cre
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5871)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /note="chicken beta-actin promoter"
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1737..2765
                     /label=Cre
                     /note="site-specific recombinase"
     CDS             2775..2804
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     polyA_signal    2960..3015
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3374..3390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3398..3414)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3422..3452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3467..3488)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3546..3742
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    3748..3882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4121..4709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4883..5740)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5741..5845)
                     /label=AmpR promoter

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