Basic Vector Information
The pWHERE plasmid was designed for studies of temporal expression and tissue distribution of your promoter of interest, cloned within an insulated LacZ cassette, in transgenic mice and rats.
- Vector Name:
- pWHERE
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9944 bp
- Replication origin:
- ori
- Source/Author:
- InvivoGen
- Selection Marker:
- lacZ
- Copy Number:
- High copy number
- Cloning Method:
- Enzyme Cut
- Expression Method:
- Transient
pWHERE vector Map
pWHERE vector Sequence
LOCUS pWHERE. 9944 bp DNA circular SYN 06-APR-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pWHERE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9944)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 9944)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9944
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(1..2032)
/label=H19 ins
/note="H19 ins"
/note="mH19 insulators on either side of the lacZ
transcription unit. Both insulators are expected to protect
the integrated transcriptional LacZ unit from negative as
well as positive influences from neighboring sequences.
Insulator elements can be functionally identified by their
ability to shield promoters from regulators in a
position-dependent manner or by their ability to protect
adjacent transgenes from position effects. The fragment of
the differential methylated region (DMD) located between
the mouse Igf2 and H19 acts as a powerful insulator. The
enhancer blocking activity of the DMD fragment is dependent
upon four responsive elements to the vertebrate
enhancer-blocking protein CTCF. Two mouse DMD fragments
have been introduced in opposite orientation in the pWHERE
plasmid to insulate your promoter of interest cloned
upstream of the new CpG-free LacZ gene from the 5' and 3'
adjacent regions at the integrated site in transgenic
mice."
CDS 2302..2322
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 5170..5742
/label=EF-1-alpha poly(A) signal
/note="human EF-1-alpha polyadenylation signal"
misc_feature complement(5750..7781)
/label=H19 ins
/note="H19 ins"
/note="mH19 insulators on either side of the lacZ
transcription unit. Both insulators are expected to protect
the integrated transcriptional LacZ unit from negative as
well as positive influences from neighboring sequences.
Insulator elements can be functionally identified by their
ability to shield promoters from regulators in a
position-dependent manner or by their ability to protect
adjacent transgenes from position effects. The fragment of
the differential methylated region (DMD) located between
the mouse Igf2 and H19 acts as a powerful insulator. The
enhancer blocking activity of the DMD fragment is dependent
upon four responsive elements to the vertebrate
enhancer-blocking protein CTCF. Two mouse DMD fragments
have been introduced in opposite orientation in the pWHERE
plasmid to insulate your promoter of interest cloned
upstream of the new CpG-free LacZ gene from the 5' and 3'
adjacent regions at the integrated site in transgenic
mice."
rep_origin complement(7877..8465)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8639..9496)
/label=AmpR
/note="beta-lactamase"
promoter complement(9497..9601)
/label=AmpR promoter
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