Basic Vector Information
pLVX-EF1α-IRES-mCherry, pLVX-EF1a-IRES-mCherry, pLVX-EF1alpha-IRES-mCherry
Basic Vector Information | |||
---|---|---|---|
Vector Name | pLVX-EF1α-IRES-mCherry | Antibiotic Resistance | Ampicillin |
Length | 8904 bp | Type | Viral Expression & Packaging Vectors |
Source | Clontech | Selection Marker | mCherry |
Copy Number | High copy number | Promoter | EF1α/ EF1a |
Cloning Method | Enzyme Cut | 5' Primer | EF1a Forward: TCAAGCCTCAGACAGTGGTTC |
3' Primer | IRES-R: CCTCACATTGCCAAAAGACG | Expression Method | Constiutive, Stable |
pLVX-EF1α-IRES-mCherry vector Vector Map
pLVX-EF1α-IRES-mCherry vector Sequence
LOCUS Exported 8904 bp ds-DNA circular SYN 25-NOV-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLVX-EF1-alpha-IRES-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8904) AUTHORS caoheibi TITLE Direct Submission JOURNAL Exported Saturday, December 5, 2020 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8904 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /note="3' LTR" /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 2028..2143 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2338..3519 /note="EF-1-alpha promoter" /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2568..3510 /note="EF-1-alpha intron A" /note="intron upstream of the start codon of human EF-1-alpha" misc_feature 3575..4147 /note="IRES" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3598..4149 /note="IRES" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 4149..4859 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="mCherry" /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 4873..5461 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(5344..5355) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" LTR 5668..6301 /note="3' LTR" /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6429..6445) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 6453..6469 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6477..6507) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 6522..6543 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6831..7419) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7590..8450) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8451..8555) /gene="bla" /note="AmpR promoter" polyA_signal 8603..8737 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal"
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