Basic Vector Information
pLVX-EF1α-IRES-mCherry, pLVX-EF1a-IRES-mCherry, pLVX-EF1alpha-IRES-mCherry
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | pLVX-EF1α-IRES-mCherry | Antibiotic Resistance | Ampicillin |
| Length | 8904 bp | Type | Viral Expression & Packaging Vectors |
| Source | Clontech | Selection Marker | mCherry |
| Copy Number | High copy number | Promoter | EF1α/ EF1a |
| Cloning Method | Enzyme Cut | 5' Primer | EF1a Forward: TCAAGCCTCAGACAGTGGTTC |
| 3' Primer | IRES-R: CCTCACATTGCCAAAAGACG | Expression Method | Constiutive, Stable |
pLVX-EF1α-IRES-mCherry vector Vector Map
pLVX-EF1α-IRES-mCherry vector Sequence
LOCUS Exported 8904 bp ds-DNA circular SYN 25-NOV-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1-alpha-IRES-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8904)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8904
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/note="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2028..2143
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/note="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/note="EF-1-alpha intron A"
/note="intron upstream of the start codon of human
EF-1-alpha"
misc_feature 3575..4147
/note="IRES"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 3598..4149
/note="IRES"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4149..4859
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/note="mCherry"
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 4873..5461
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(5344..5355)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
LTR 5668..6301
/note="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6429..6445)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6453..6469
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6477..6507)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6522..6543
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6831..7419)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7590..8450)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8451..8555)
/gene="bla"
/note="AmpR promoter"
polyA_signal 8603..8737
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
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