pEE12.4 vector (V012681)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012681 pEE12.4 In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • pEE12.4
      • Length:
      • 7569 bp
      • Type:
      • Mammalian Expression Vectors
      • Source/Author:
      • Lonza biologics
      • Selection Marker:
      • GS(Glutamine Synthetase)
      • Copy Number:
      • High copy number
      • Promoter:
      • CMV
      • Cloning Method:
      • Enzyme Cut
      • 5' Primer:
      • CMV fwd
      • Expression Method:
      • Constiutive, Stable

pEE12.4 vector Vector Map

pEE12.47569 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600690072007500SV40 poly(A) signaloriAmpRAmpR promoterT7Te terminatorSV40 promoterGLULsmall t intronSV40 NLSSV40 poly(A) signalCMV enhancerCMV promoterCMV intron A

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEE12.4 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pEE12.4.        7569 bp DNA     circular SYN 23-SEP-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pEE12.4.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7569)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    116..237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(999..1587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1761..2618)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2619..2723)
                     /label=AmpR promoter
     terminator      2892..2919
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3002..3331
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3370..4488
                     /gene="GLUL"
                     /label=GLUL
                     /note="Glutamine synthetase from Acomys cahirinus.
                     Accession#: Q9QY94"
     intron          4623..4688
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4818..4838
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5263..5397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        5966..6345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6346..6549
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          6685..7511
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"