Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | pEE12.4 | Length | 7569 bp |
| Type | Mammalian Expression Vectors | Source | Lonza biologics |
| Selection Marker | GS(Glutamine Synthetase) | Copy Number | High copy number |
| Promoter | CMV | Cloning Method | Enzyme Cut |
| 5' Primer | CMV fwd | Expression Method | Constiutive, Stable |
pEE12.4 vector Vector Map
pEE12.4 vector Sequence
LOCUS Exported 7569 bp ds-DNA circular SYN 23-SEP-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pEE12.4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7569)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7569
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 116..237
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(999..1587)
/direction=LEFT
/note="pEE6 ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1758..2618)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2619..2723)
/gene="bla"
/note="AmpR promoter"
terminator 2892..2919
/note="T7Te terminator"
/note="phage T7 early transcription terminator"
promoter 3002..3331
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 3182..3317
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3370..4491
/codon_start=1
/note="GS"
/translation="MATSASSHLNKNIKQMYLCLPQGEKVQAMYIWVDGTGEGLRCKTR
TLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRRDPNKLVFCEVFKY
NRKPAETNLRHSCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYC
GVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR
FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKHIEEAIEKLSKRH
RYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDR
RPSANCDPFAVTEAIVRTCLLNETGDEPFQYKN"
intron 4623..4688
/note="small t intron"
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4818..4838
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
polyA_signal 5263..5397
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
enhancer 5966..6345
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 6346..6549
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
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