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pEE12.4 vector (V012681#)

Basic Vector Information

Basic Vector Information
Vector Name pEE12.4 Length 7569 bp
Type Mammalian Expression Vectors Source Lonza biologics
Selection Marker GS(Glutamine Synthetase) Copy Number High copy number
Promoter CMV Cloning Method Enzyme Cut
5' Primer CMV fwd Expression Method Constiutive, Stable

pEE12.4 vector Vector Map

pEE12.4 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                7569 bp ds-DNA     circular SYN 23-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pEE12.4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    116..237
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(999..1587)
                     /direction=LEFT
                     /note="pEE6 ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1758..2618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2619..2723)
                     /gene="bla"
                     /note="AmpR promoter"
     terminator      2892..2919
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     promoter        3002..3331
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      3182..3317
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             3370..4491
                     /codon_start=1
                     /note="GS"
                     /translation="MATSASSHLNKNIKQMYLCLPQGEKVQAMYIWVDGTGEGLRCKTR
                     TLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRRDPNKLVFCEVFKY
                     NRKPAETNLRHSCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYC
                     GVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR
                     FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKHIEEAIEKLSKRH
                     RYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDR
                     RPSANCDPFAVTEAIVRTCLLNETGDEPFQYKN"
     intron          4623..4688
                     /note="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4818..4838
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    5263..5397
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     enhancer        5966..6345
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6346..6549
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"

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