Basic Vector Information
Basic Vector Information | |||
---|---|---|---|
Vector Name | pEE12.4 | Length | 7569 bp |
Type | Mammalian Expression Vectors | Source | Lonza biologics |
Selection Marker | GS(Glutamine Synthetase) | Copy Number | High copy number |
Promoter | CMV | Cloning Method | Enzyme Cut |
5' Primer | CMV fwd | Expression Method | Constiutive, Stable |
pEE12.4 vector Vector Map
pEE12.4 vector Sequence
LOCUS Exported 7569 bp ds-DNA circular SYN 23-SEP-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEE12.4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7569) AUTHORS . TITLE Direct Submission JOURNAL Exported Saturday, December 5, 2020 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7569 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 116..237 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(999..1587) /direction=LEFT /note="pEE6 ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1758..2618) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2619..2723) /gene="bla" /note="AmpR promoter" terminator 2892..2919 /note="T7Te terminator" /note="phage T7 early transcription terminator" promoter 3002..3331 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 3182..3317 /note="SV40 ori" /note="SV40 origin of replication" CDS 3370..4491 /codon_start=1 /note="GS" /translation="MATSASSHLNKNIKQMYLCLPQGEKVQAMYIWVDGTGEGLRCKTR TLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRRDPNKLVFCEVFKY NRKPAETNLRHSCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYC GVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKHIEEAIEKLSKRH RYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDR RPSANCDPFAVTEAIVRTCLLNETGDEPFQYKN" intron 4623..4688 /note="small t intron" /note="SV40 (simian virus 40) small t antigen intron" CDS 4818..4838 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /note="SV40 NLS" /translation="PKKKRKV" polyA_signal 5263..5397 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" enhancer 5966..6345 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 6346..6549 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter"
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