Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V012681 | pEE12.4 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pEE12.4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEE12.4 vector Sequence
LOCUS pEE12.4. 7569 bp DNA circular SYN 23-SEP-2020 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pEE12.4. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7569) TITLE Direct Submission REFERENCE 2 (bases 1 to 7569) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..7569 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal 116..237 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(999..1587) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1761..2618) /label=AmpR /note="beta-lactamase" promoter complement(2619..2723) /label=AmpR promoter terminator 2892..2919 /label=T7Te terminator /note="phage T7 early transcription terminator" promoter 3002..3331 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 3370..4488 /gene="GLUL" /label=GLUL /note="Glutamine synthetase from Acomys cahirinus. Accession#: Q9QY94" intron 4623..4688 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4818..4838 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 5263..5397 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" enhancer 5966..6345 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 6346..6549 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 6685..7511 /label=CMV intron A /note="human cytomegalovirus intron A (Chapman et al., 1991)"