pAdDeltaF6 vector (V000011)

Price Information

Cat No. Plasmid Name Availability Add to cart
V000011 pAdDeltaF6 In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • pAdDeltaF6
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 15420 bp
      • Type:
      • AAV
      • Copy Number:
      • Low Copy

pAdDeltaF6 vector Vector Map

pAdDeltaF615420 bp70014002100280035004200490056006300700077008400910098001050011200119001260013300140001470015400SK primerT3M13 revlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterpBRforEcotet promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pAdDeltaF6 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               15420 bp ds-DNA     circular SYN 19-OCT-2021
DEFINITION  AAV helper plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    pAdDeltaF6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15420)
  TITLE     AAV packaging plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
FEATURES             Location/Qualifiers
     source          1..15420
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(142..158)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(142..158)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(195..215)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(195..213)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(234..250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(234..250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(247..269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    258..274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(282..312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    327..348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(465..482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(636..1224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(716..735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1395..2255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2018..2037
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2256..2360)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2428..2446
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2473..2501
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"