Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V000011 | pAdDeltaF6 | In stock, instant shipping |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pAdDeltaF6
- Antibiotic Resistance:
- Ampicillin
- Length:
- 15420 bp
- Type:
- AAV
- Replication origin:
- ori
- Copy Number:
- Low Copy
- Promoter:
- tet
pAdDeltaF6 vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pAdDeltaF6 vector Sequence
LOCUS V000011 15420 bp DNA circular SYN 19-OCT-2021
DEFINITION Exported.
ACCESSION V000011
VERSION V000011
KEYWORDS pAdDeltaF6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 15420)
TITLE AAV packaging plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 15420)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 15420)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..15420
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(142..158)
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(195..213)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(234..250)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(258..274)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(282..312)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(327..348)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(465..482)
/label="L4440"
/note="L4440 vector, forward primer"
rep_origin complement(636..1224)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1398..2255)
/label="AmpR"
/note="beta-lactamase"
promoter complement(2256..2360)
/label="AmpR promoter"
primer_bind 2428..2446
/label="pBRforEco"
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 2473..2501
/label="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
repeat_region 3013..3115
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
CDS 3425..3808
/note="Early 4 ORF1 protein from Human adenovirus C
serotype 2. Accession#: P03242"
/label="Early 4 ORF1 protein"
CDS 4248..4595
/note="Early 4 ORF3 protein from Human adenovirus C
serotype 2. Accession#: P03241"
/label="Early 4 ORF3 protein"
CDS 4874..5755
/note="Early 4 ORF6 protein from Human adenovirus C
serotype 2. Accession#: P03239"
/label="Early 4 ORF6 protein"
CDS complement(6167..7909)
/note="Fiber protein from Human adenovirus C serotype 5.
Accession#: P11818"
/label="Fiber protein"
CDS complement(8444..9124)
/gene="L4"
/label="Pre-hexon-linking protein VIII"
/note="Pre-hexon-linking protein VIII from Human adenovirus
C serotype 5. Accession#: P24936"
CDS complement(9811..12231)
/gene="L4"
/label="Shutoff protein"
/note="Shutoff protein from Human adenovirus C serotype 5.
Accession#: P24933"
CDS 12260..13846
/gene="DBP"
/label="DNA-binding protein"
/note="DNA-binding protein from Human adenovirus C serotype
5. Accession#: P03265"