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pENTR1A-Huwe1 vector (V000614)

Basic Vector Information

Vector Name:
pENTR1A-Huwe1
Antibiotic Resistance:
Kanamycin
Length:
16589 bp
Type:
Gateway Entry
Replication origin:
ori
Copy Number:
Low Copy
Cloning Method:
Gateway Cloning
5' Primer:
hHuwe1-R GCCAAATGTTGTAGCCGAGT)(
3' Primer:
hHuwe1-F (TATCAGGACTGCCCACCATT)

pENTR1A-Huwe1 vector Vector Map

pENTR1A-Huwe116589 bp800160024003200400048005600640072008000880096001040011200120001280013600144001520016000attL1attL2KanRorirrnB T1 terminatorrrnB T2 terminatorpENTR-F

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pENTR1A-Huwe1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_17483       16589 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Cook, Jean
  TITLE     Huwe1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16589)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 16589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..16589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(14461..14560)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             14683..15489
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      15582..16170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      16335..16421
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      16513..16540
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     16548..16571
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"

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