Basic Vector Information
- Vector Name:
- 1.3xWT HBV-Luciferase
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6656 bp
- Type:
- Mammalian Expression, Luciferase
- Replication origin:
- ori
- Promoter:
- SP6
1.3xWT HBV-Luciferase vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
1.3xWT HBV-Luciferase vector Sequence
LOCUS 1.3xWT_HBV-Lucif 6656 bp DNA circular SYN 13-MAY-2021
DEFINITION The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1.
Luciferase inserted within Core ORF. 2. Luciferase is regulated by
EnhII - Core promoter. 3. The functional luciferase ATG is of Core.
ACCESSION .
VERSION .
KEYWORDS 1.3xWT HBV-Luciferase.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6656)
AUTHORS Doitsh G, Shaul Y
TITLE Enhancer I predominance in hepatitis B virus gene expression.
JOURNAL Mol Cell Biol. 2004 Feb;24(4):1799-808.
PUBMED 14749394
REFERENCE 2 (bases 1 to 6656)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6656)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell
Biol."; date: "2004-02"; volume: "24(4)"; pages: "1799-808"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6656
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 355..816
/gene="X"
/label=X
/note="Protein X from Hepatitis B virus genotype A2 subtype
adw2 (strain Rutter 1979). Accession#: P69713"
CDS 969..2618
/label=luciferase
/note="firefly luciferase"
CDS 3317..3778
/gene="X"
/label=X
/note="Protein X from Hepatitis B virus genotype A2 subtype
adw2 (strain Rutter 1979). Accession#: P69713"
promoter complement(3981..3999)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4017..4033)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4041..4057)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4065..4095)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4110..4131)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4248..4265)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4419..5007)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5181..6038)
/label=AmpR
/note="beta-lactamase"
promoter complement(6039..6143)
/label=AmpR promoter
primer_bind 6211..6229
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6267..6289)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6389..6408
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6617..6633
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 6640..6656
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
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