Basic Vector Information
- Vector Name:
- pEGFP hsp70
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6888 bp
- Type:
- Mammalian Expression
- Replication origin:
- ori
- Selection Marker:
- Neomycin (select with G418)
- Copy Number:
- High Copy
- Promoter:
- SV40
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- EGFP-C primer
- 3' Primer:
- SV40pA-R
pEGFP hsp70 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEGFP hsp70 vector Sequence
LOCUS V000915 6888 bp DNA circular SYN 13-MAY-2021
DEFINITION Exported.
ACCESSION V000915
VERSION V000915
KEYWORDS pEGFP hsp70.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6888)
AUTHORS Zeng XC, Bhasin S, Wu X, Lee JG, Maffi S, Nichols CJ, Lee KJ, Taylor
JP, Greene LE, Eisenberg E
TITLE Hsp70 dynamics in vivo: effect of heat shock and protein
aggregation.
JOURNAL J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-5000.
PUBMED 15367583
REFERENCE 2 (bases 1 to 6888)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6888)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
2004 Oct 1. 117(Pt 21):4991-5000."
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6888
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 54..357
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 358..561
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 606..1322
/label="EGFP"
/note="enhanced GFP"
CDS 1347..3269
/gene="HSPA1A"
/label="Heat shock 70 kDa protein 1A"
/note="Heat shock 70 kDa protein 1A from Homo sapiens.
Accession#: P0DMV8"
polyA_signal 3669..3790
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3797..4252)
/direction=LEFT
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4279..4383
/label="AmpR promoter"
promoter 4385..4742
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 4777..5568
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
primer_bind complement(5759..5778)
/label="TK-pA-R"
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5803..5850
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 6179..6767
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 6882..6888
/label="CMV"
/note="Human cytomegalovirus immediate early
enhancer/promoter"
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