Basic Vector Information
pAAV2/2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pAAV2/2 vector Sequence
LOCUS Exported 7468 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION AAV packaging plasmid expressing Rep/Cap genes for production of
serotype 2.
ACCESSION .
VERSION .
KEYWORDS pAAV2/2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7468)
TITLE AAV Packaging
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7468)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..7468
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 132..151
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 385..402
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 519..540
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 555..585
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 593..609
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 598..620
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 617..633
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 617..633
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 683..699
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 683..699
/label=pBluescriptSK
/note="For pBluescript vector"
protein_bind 713..760
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 4850..4861
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
primer_bind complement(5336..5353)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5336..5352)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5345..5367)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin complement(5493..5948)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5631..5652)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5842..5861
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 5975..6079
/gene="bla"
/label=AmpR promoter
CDS 6080..6940
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6298..6317)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin join(7111..7468,1..231)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.