pAAV2/2 vector (V001085)

Basic Vector Information

      • Vector Name:
      • pAAV2/2
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7468 bp
      • Type:
      • Mammalian Expression, AAV
      • Copy Number:
      • High Copy
      • Promoter:
      • p5
      • 5' Primer:
      • M13 Reverse

pAAV2/2 vector Vector Map

pAAV2/27468 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069007200pBR322ori-FL4440CAP binding sitelac promoterlac operatorM13 revSK primerFRTWELQut siteM13 Forwardf1 oriAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pAAV2/2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                7468 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  AAV packaging plasmid expressing Rep/Cap genes for production of 
            serotype 2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7468)
  TITLE     AAV Packaging
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7468)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     132..151
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /note="L4440 vector, forward primer"
     protein_bind    519..540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        555..585
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     598..620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     617..633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
     primer_bind     617..633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     683..699
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
     primer_bind     683..699
                     /note="For pBluescript vector"
     protein_bind    713..760
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4850..4861
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
     primer_bind     complement(5336..5353)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
     primer_bind     complement(5336..5352)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
     primer_bind     complement(5345..5367)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5493..5948)
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5631..5652)
                     /note="F1 origin, forward primer"
     primer_bind     5842..5861
                     /note="F1 origin, reverse primer"
     promoter        5975..6079
                     /label=AmpR promoter
     CDS             6080..6940
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     primer_bind     complement(6298..6317)
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7111..7468,1..231)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

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