pAC-BETAipi vector (V001187)

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Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V001187	pAC-BETAipi	In stock, 1 week for quality controls	Buy one, get one free!

Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Vector Name:: pAC-BETAipi

Antibiotic Resistance:: Chloramphenicol

Length:: 9259 bp

Type:: low copy number bacterial cloning vector

Replication origin:: p15A ori

Copy Number:: Low Copy

Promoter:: endogenous promoter

Cloning Method:: Restriction Enzyme

pAC-BETAipi vector Map

Copy Sequence View Map

Plasmid Protocol

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5. Store the plasmid at -20 ℃.

6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it

General Plasmid Transform Protocol

1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.

2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.

3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.

4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.

5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.

pAC-BETAipi vector Sequence

LOCUS       V001187                 9259 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001187
VERSION     V001187
KEYWORDS    pAC-BETAipi
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A study in scarlet: enzymes of ketocarotenoid biosynthesis in the
            flowers of Adonis aestivalis.
  JOURNAL   Plant J. 2005 Feb;41(3):478-92.
   PUBMED   15659105
REFERENCE   2  (bases 1 to 9259)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2005-02"; volume: "41(3)"; pages: "478-92"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(220..322)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        1505..1533
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1993..2913
                     /gene="crtE"
                     /label="Geranylgeranyl diphosphate synthase"
                     /note="Geranylgeranyl diphosphate synthase from
                     Pseudescherichia vulneris. Accession#: P22873"
     CDS             2979..4019
                     /gene="fni"
                     /label="Isopentenyl-diphosphate delta-isomerase"
                     /note="Isopentenyl-diphosphate delta-isomerase from
                     Pseudescherichia vulneris. Accession#: Q01335"
     CDS             4449..5606
                     /gene="crtY"
                     /label="Lycopene beta-cyclase"
                     /note="Lycopene beta-cyclase from Pseudescherichia
                     vulneris. Accession#: Q01331"
     CDS             5606..7081
                     /gene="crtI"
                     /label="Phytoene desaturase (lycopene-forming)"
                     /note="Phytoene desaturase (lycopene-forming) from
                     Pseudescherichia vulneris. Accession#: P22871"
     CDS             complement(join(8822..9259,1..219))
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"