Basic Vector Information
pAAV2/9n vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pAAV2/9n vector Sequence
LOCUS Exported 7330 bp ds-DNA circular SYN 16-DEC-2021
DEFINITION AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION .
VERSION .
KEYWORDS pAAV2/9n
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7330)
TITLE AAV packaging plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7330)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..7330
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 122..144
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 136..153
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 137..153
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 163..182
/label=T7
/note="T7 promoter, forward primer"
promoter 163..181
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 210..226
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 211..227
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 4216..4227
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
primer_bind complement(4679..4699)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4679..4697)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4718..4734)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4718..4734)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4731..4753)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4742..4758
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4766..4796)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4811..4832
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4949..4966)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5120..5708)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5200..5219)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5879..6739)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6502..6521
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6740..6844)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6870..7325)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7007..7028)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7219..7238
/label=F1ori-R
/note="F1 origin, reverse primer"
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