pAAV2/9n vector (V001257)

Basic Vector Information

      • Vector Name:
      • pAAV2/9n
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7330 bp
      • Type:
      • AAV
      • Promoter:
      • Truncated P5
      • 5' Primer:
      • KS primer

pAAV2/9n vector Vector Map

pAAV2/9n7330 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069007200In lacZ geneT7pBluescriptKSWELQut siteT3M13 revlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterf1 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pAAV2/9n vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                7330 bp ds-DNA     circular SYN 16-DEC-2021
DEFINITION  AAV packaging plasmid expressing Rep/Cap genes.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7330)
  TITLE     AAV packaging plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7330)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     122..144
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     136..153
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
     primer_bind     137..153
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
     primer_bind     163..182
                     /note="T7 promoter, forward primer"
     promoter        163..181
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     210..226
                     /note="For pBluescript vector"
     primer_bind     211..227
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
     CDS             4216..4227
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
     primer_bind     complement(4679..4699)
                     /note="T3 promoter, forward primer"
     promoter        complement(4679..4697)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4718..4734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
     primer_bind     complement(4718..4734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4731..4753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4742..4758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4766..4796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4811..4832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4949..4966)
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5120..5708)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     primer_bind     complement(5200..5219)
                     /note="pBR322 origin, forward primer"
     CDS             complement(5879..6739)
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     primer_bind     6502..6521
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6740..6844)
                     /label=AmpR promoter
     rep_origin      complement(6870..7325)
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7007..7028)
                     /note="F1 origin, forward primer"
     primer_bind     7219..7238
                     /note="F1 origin, reverse primer"

This page is informational only.