pEGFP-rNFM vector (V001415)

MSDS

Basic Vector Information

Vector Name:: pEGFP-rNFM

Antibiotic Resistance:: Kanamycin

Length:: 7464 bp

Type:: Mammalian Expression

Replication origin:: ori

Selection Marker:: Neomycin (select with G418)

Copy Number:: High Copy

Promoter:: CMV

Cloning Method:: Restriction Enzyme

5' Primer:: multiple primers

3' Primer:: multiple primers

pEGFP-rNFM vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEGFP-rNFM vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V001415                 7464 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001415
VERSION     V001415
KEYWORDS    pEGFP-rNFM.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Wang L, Ho CL, Sun D, Liem RK, Brown A
  TITLE     Rapid movement of axonal neurofilaments interrupted by prolonged
            pauses.
  JOURNAL   Nat Cell Biol. 2000 Mar;2(3):137-41.
   PUBMED   10707083
REFERENCE   2  (bases 1 to 7464)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7464)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell
            Biol."; date: "2000-03"; volume: "2(3)"; pages: "137-41"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1456..3939
                     /gene="Nefm"
                     /label="Neurofilament medium polypeptide"
                     /note="Neurofilament medium polypeptide from Rattus
                     norvegicus. Accession#: P12839"
     polyA_signal    4252..4373
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4380..4835)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4862..4966
                     /label="AmpR promoter"
     promoter        4968..5325
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5360..6151
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(6342..6361)
                     /label="TK-pA-R"
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6386..6433
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6762..7350
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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