pOTENT-1 vector (V001455)

Price Information

Cat No. Plasmid Name Availability Add to cart
V001455 pOTENT-1 In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Adenoviral pOTENT-1 shuttle vector with kanamycin selection and CMV promoter and C-terminal FLAG and His tags.

      • Vector Name:
      • pOTENT-1
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 7509 bp
      • Type:
      • Mammalian Expression Vectors
      • Selection Marker:
      • Puromycin
      • Copy Number:
      • Low copy number
      • Promoter:
      • CMV
      • Cloning Method:
      • Enzyme Cut
      • 5' Primer:
      • T7 promoter
      • 3' Primer:
      • pOT-R
      • Fusion Tag:
      • FLAG/His
      • Expression Method:
      • Constiutive, Stable / Transient

pOTENT-1 vector Vector Map

pOTENT-17509 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600690072007500ITRAd5 PsiCMV enhancerCMV promoterT7 promoterFLAG6xHisSV40 poly(A) signalIXSV40 promoterPuroRbGH poly(A) signalEarly 4 ORF1 protein from Human adenovirus C serotype 2. Accession#: P03242ITRoriNeoR/KanRM13 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pOTENT-1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pOTENT-1.        7509 bp DNA     circular SYN 08-SEP-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pOTENT-1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7509)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   21..123
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     211..361
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        419..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        954..972
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1073..1096
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1106..1123
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1294..1415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1509..1928
                     /gene="IX"
                     /label=IX
                     /note="Hexon-interlacing protein from Human adenovirus C 
                     serotype 5. Accession#: P03281"
     promoter        2342..2671
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2729..3325
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3329..3553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3771..4154)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
     repeat_region   4464..4566
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(4838..5426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             6258..7049
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7100..7480)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"