pCOLD-MazF-K56A vector (V001461)

LOCUS       V001461                 6090 bp    DNA     circular SYN 25-JUL-2018
DEFINITION  Exported.
ACCESSION   V001461
VERSION     V001461
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   TaKaRa
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..81
                     /label="cspA promoter"
                     /note="promoter of the E. coli cold shock protein cspA gene
                     (Mitta et al., 1997)"
     protein_bind    84..100
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     5'UTR           122..255
                     /label="cspA 5'UTR"
                     /note="5'UTR of the E. coli cold shock protein cspA gene
                     (Mitta et al., 1997)"
     CDS             256..270
                     /label="TEE"
                     /note="translation enhancing element for E. coli (Qing et
                     al., 2004)"
     CDS             271..288
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             289..1584
                     /label="Trigger Factor"
                     /note="ribosome-associated chaperone from E. coli (Hoffmann
                     et al., 2010)"
     CDS             1594..1617
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             1627..1644
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             1651..1662
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1666..1998
                     /gene="mazF"
                     /label="Endoribonuclease toxin MazF"
                     /note="Endoribonuclease toxin MazF from Escherichia coli
                     (strain K12). Accession#: P0AE70"
     3'UTR           2051..2195
                     /label="cspA 3'UTR"
                     /note="3'UTR of the E. coli cold shock protein cspA gene
                     (Mitta et al., 1997)"
     rep_origin      complement(2394..2849)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2949..3053
                     /label="AmpR promoter"
     CDS             3054..3911
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4085..4673
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    complement(4811..4832)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4848..5927)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5928..6005)
                     /label="lacI promoter"