yrbE1AB vector (V001470)

LOCUS       V001470                 6299 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001470
VERSION     V001470
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Joshi SM, Pandey AK, Capite N, Fortune SM, Rubin EJ, Sassetti CM.
  TITLE     Characterization of mycobacterial virulence genes through genetic
            interaction mapping
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 103 (31), 11760-11765 (2006)
   PUBMED   16868085
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Joshi SM, Pandey AK, Capite NM, Fortune SM, Rubin EJ, Sassetti CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2006) Molecular Genetics and Microbiology,
            University of Massachusetts Medical School, 55 Lake Ave. North,
            Worcester, MA 01655, USA
REFERENCE   3  (bases 1 to 6299)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6299)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2006"; volume: "103"; issue: "31"; pages:
            "11760-11765"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-JUN-2006) Molecular Genetics and Microbiology, University of
            Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA
            01655, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..394
                     /note="promoter from Mycobacterium tuberculosis groEL2
                     gene"
                     /regulatory_class="promoter"
     CDS             446..1243
                     /codon_start=1
                     /gene="yrbE1A"
                     /product="YrbE1A"
                     /label="yrbE1A"
                     /protein_id="ABG72765.1"
                     /translation="MTTSTTLGGYVRDQLQTPLTLVGGFFRMCVLTGKALFRWPFQWRE
                     FILQCWFIMRVGFLPTIMVSIPLTVLLIFTLNILLAQFGAADISGSGAAIGAVTQLGPL
                     TTVLVVAGAGSTAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVLASMLVATLLNGL
                     VITVGLVGGFLFGVYLQNVSGGAYLATLTLITGLPEVVIATIKAATFGLIAGLVGCYRG
                     LTVRGGSKGLGTAVNETVVLCVIALFAVNVILTTIGVRFGTGR"
     gene            446..1243
                     /gene="yrbE1A"
                     /label="yrbE1A"
     CDS             2118..2144
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     terminator      2200..2246
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2298..3293)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     rep_origin      3632..4220
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      4379..6273
                     /label="mycobacterial origin of replication from pAL5000"
                     /note="mycobacterial origin of replication from pAL5000"