YFV-C2opt-NS2mut vector (V001486)

LOCUS       40924_49677       10928 bp DNA     circular SYN 18-DEC-2018
DEFINITION  VEEV replicon vector YFV-C2opt-NS2mut, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Trans-packaging of Yellow Fever virus replicons: an approach for 
            flaviviral antigen production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2005) Department of Microbiology and Immunology, 
            University of Texas Medical Branch, 301 University Boulevard, 
            Galveston, TX 77550-1019, USA
REFERENCE   3  (bases 1 to 10928)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10928)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2005) Department of Microbiology and Immunology, University 
            of Texas Medical Branch, 301 University Boulevard, Galveston, TX 
            77550-1019, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..45
                     /note="5'UTR of Venezuelan equine encephalitis virus VEE
                     TC83"
     misc_feature    46..1650
                     /note="nsP1 of Venezuelan equine encephalitis virus VEE
                     TC83"
     RBS             1536..1544
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    1651..4032
                     /note="nsP2 of Venezuelan equine encephalitis virus VEE
                     TC83"
     misc_difference 3866
                     /replace="a"
                     /note="A->T mutation from TC83 sequence produce Leu mutant
                     in nsP2"
     misc_feature    4033..5703
                     /note="nsP3 of Venezuelan equine encephalitis virus VEE
                     TC83"
     misc_feature    5704..7524
                     /note="nsP4 of Venezuelan equine encephalitis virus VEE
                     TC83"
     misc_feature    7502..7546
                     /note="subgenomic promoter of Venezuelan equine
                     encephalitis virus VEE TC83"
     misc_feature    7571..7933
                     /note="Yellow fever virus 17D capsid protein (synthetic, 
                     codon-optimized)"
     misc_feature    7934..7993
                     /note="first 20 aa from PR of Yellow fever virus 17D
                     (synthetic, codon-optimized)"
     misc_feature    7997..8041
                     /note="subgenomic promoter of Venezuelan equine
                     encephalitis virus VEE TC83 (second copy)"
     CDS             8071..8667
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    8747..9102
                     /note="3'UTR of Venezuelan equine encephalitis virus VEE
                     TC83"
     promoter        9166..9270
                     /label=AmpR promoter
     CDS             9271..10128
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      10302..10890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"