Basic Vector Information
- Vector Name:
- YCplac22 5-1.2-FLuciferase
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7428 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Belmont BJ, Niles JC.
- Promoter:
- TRP1
YCplac22 5-1.2-FLuciferase vector Vector Map
YCplac22 5-1.2-FLuciferase vector Sequence
LOCUS 40924_49467 7428 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector YCplac22 5-1.2-FLuciferase, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7428) AUTHORS Belmont BJ, Niles JC. TITLE Inducible Control of Subcellular RNA Localization Using a Synthetic Protein-RNA Aptamer Interaction JOURNAL PLoS ONE 7 (10), E46868 (2012) PUBMED 23056498 REFERENCE 2 (bases 1 to 7428) AUTHORS Belmont BJ, Niles JC. TITLE Direct Submission JOURNAL Submitted (12-SEP-2012) Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139, USA REFERENCE 3 (bases 1 to 7428) TITLE Direct Submission REFERENCE 4 (bases 1 to 7428) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; date: "2012"; volume: "7"; issue: "10"; pages: "E46868" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (12-SEP-2012) Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..7428 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 17..33 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 678..2327 /codon_start=1 /label=luciferase /note="firefly luciferase" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" primer_bind complement(2632..2648) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature complement(3407..4569) /label=CEN/ARS /note="S. cerevisiae CEN4 centromere fused to the autonomously replicating sequence ARS1/ARS416" promoter complement(5083..5184) /label=TRP1 promoter promoter 5290..5394 /label=AmpR promoter CDS 5395..6252 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6426..7014 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7302..7323 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 7338..7368 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7376..7392 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7400..7416 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
This page is informational only.