Basic Vector Information
- Vector Name:
- WRKY29-LUC-NOS-At4g23550
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7292 bp
- Type:
- Control vector
- Replication origin:
- ori
- Source/Author:
- Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Boller T, Ausubel FM, Sheen J.
WRKY29-LUC-NOS-At4g23550 vector Map
WRKY29-LUC-NOS-At4g23550 vector Sequence
LOCUS 40924_49377 7292 bp DNA circular SYN 18-DEC-2018 DEFINITION Control vector WRKY29-LUC-NOS-At4g23550, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7292) AUTHORS Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Boller T, Ausubel FM, Sheen J. TITLE MAP kinase signalling cascade in Arabidopsis innate immunity JOURNAL Nature 415 (6875), 977-983 (2002) PUBMED 11875555 REFERENCE 2 (bases 1 to 7292) AUTHORS Sheen J. TITLE Direct Submission JOURNAL Submitted (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, USA REFERENCE 3 (bases 1 to 7292) TITLE Direct Submission REFERENCE 4 (bases 1 to 7292) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; date: "2002"; volume: "415"; issue: "6875"; pages: "977-983" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7292 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(15..31) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(39..55) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(63..93) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(108..129) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(417..1005) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1179..2036) /label=AmpR /note="beta-lactamase" promoter complement(2037..2141) /label=AmpR promoter primer_bind 2615..2631 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 2662..5228 /label=pWRKY29 /note="pWRKY29" regulatory 2667..5228 /label=WRKY29 promoter /note="WRKY29 promoter" /regulatory_class="promoter" CDS 5229..6878 /label=luciferase /note="firefly luciferase" terminator 7036..7287 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal"
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