pCVD077 vector (V001517)

LOCUS       40924_49332        6221 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD077, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 6221)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..6
                     /label=ZraI
                     /note="ZraI"
     misc_feature    4..24
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    25..30
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     31..1091
                     /note="A7120NS1-RA; right arm of the homologous
                     recombination site NS1 (alr3858_D748-alr3858_D749) into the
                     chromosome of Anabaena sp. PCC7120 derived from INSD 
                     accession NC_003272"
     misc_feature    31..38
                     /label=SwaI
                     /note="SwaI"
     misc_feature    515..520
                     /label=XbaI
                     /note="XbaI"
     rep_origin      1128..1716
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1902..2042)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    2205..2210
                     /label=EcoRV
                     /note="EcoRV"
     CDS             complement(2233..3651)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     regulatory      complement(3652..3957)
                     /note="PsacB; derived from Bacillus subtilis INSD accession
                     AAA72307"
                     /regulatory_class="promoter"
     misc_recomb     3958..4965
                     /note="A7120NS1-LA; right arm of the homologous
                     recombination site NS1 (alr3858_D748-alr3858_D749) into the
                     chromosome of Anabaena sp. PCC7120 derived from INSD 
                     accession NC_003272"
     misc_feature    4697..4702
                     /label=MfeI
                     /note="MfeI"
     misc_feature    4966..4971
                     /label=NheI
                     /note="NheI"
     misc_feature    4972..4992
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    4990..4995
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(5119..5976)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5977..6081)
                     /label=AmpR promoter
     misc_feature    6107..6112
                     /label=ZraI
                     /note="ZraI"