pCVD075 vector (V001519)

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LOCUS       V001519                 3376 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001519
VERSION     V001519
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B,
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
   PUBMED   25074377
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B,
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. (2014) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUN-2014) Division of Biological Sciences, University of
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        320..338
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    370..375
                     /label="EcoRV"
                     /note="EcoRV"
     misc_feature    complement(373..393)
                     /note="C2G; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    394..399
                     /label="AgeI"
                     /note="AgeI"
     regulatory      400..571
                     /note="Perm; upstream region of erm ORF including promoter
                     and RBS; derived from pRL692 in INSD accession AF424805"
                     /regulatory_class="promoter"
     CDS             572..1303
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     regulatory      1339..1365
                     /note="trpA T; trpA terminator from Lorist6 INSD accession
                     X98450, one nt shorter than reference; Lorist6"
                     /regulatory_class="terminator"
     misc_feature    1375..1380
                     /label="NheI"
                     /note="NheI"
     misc_feature    complement(1381..1401)
                     /note="GC; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers or Escherichia coli origins
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    1399..1404
                     /label="EcoRV"
                     /note="EcoRV"
     rep_origin      complement(1629..2217)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2391..3248)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3249..3353)
                     /label="AmpR promoter"