pCVD047 vector (V001540)

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LOCUS       40924_49217        7140 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD047, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 7140)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7140)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(124..981)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(982..1086)
                     /label=AmpR promoter
     misc_feature    1112..1117
                     /label=ZraI
                     /note="ZraI"
     misc_feature    1227..1232
                     /label=ZraI
                     /note="ZraI"
     misc_feature    1230..1250
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    1251..1256
                     /label=XbaI
                     /note="XbaI"
     regulatory      1288..1314
                     /note="trpA T; trpA terminator from Lorist6 INSD accession 
                     X98450, one nt shorter than reference; Lorist6"
                     /regulatory_class="terminator"
     CDS             complement(1650..2498)
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             complement(2488..3324)
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             complement(3354..3560)
                     /codon_start=1
                     /product="repF"
                     /label=repF
                     /note="repF-RSF1010; replication gene; derived from
                     pRL1383a in INSD accession AF403426, a derivative of 
                     RSF1010 INSD accession M28829"
                     /protein_id="AII99706.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             complement(3562..3774)
                     /codon_start=1
                     /product="repE"
                     /label=repE
                     /note="repE-RSF1010; replication gene; derived from
                     pRL1383a in INSD accession AF403426, a derivative of 
                     RSF1010 INSD accession M28829"
                     /protein_id="AII99707.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     regulatory      complement(3804..3831)
                     /note="P4; derived from pRL1383a in INSD accession
                     AF403426, a derivative of RSF1010 INSD accession M28829; 
                     the P4 promoter is under the auto-regulated control of the 
                     F repressor, whose gene is located in the same operon, 
                     providing transcription of E-F-repA-repC-operon"
                     /regulatory_class="promoter"
     CDS             complement(3838..4806)
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             complement(4803..5216)
                     /codon_start=1
                     /product="mobB"
                     /label=mobB
                     /note="mobilization gene; derived from pRL1383a in INSD 
                     accession AF403426, a derivative of RSF1010 INSD accession 
                     M28829"
                     /protein_id="AII99704.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTKQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     misc_feature    5662..5667
                     /label=AgeI
                     /note="AgeI"
     oriT            complement(6045..6132)
                     /direction=LEFT
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     regulatory      complement(6141..6172)
                     /note="P1; derived from pRL1383a in INSD accession
                     AF403426, a derivative of RSF1010 INSD accession M28829; 
                     the P1 promoter is controlled by MobC and MobA provide 
                     transcription of mobA/repB, mobB, and, probably, 
                     E-F-repA-repC-operon"
                     /regulatory_class="promoter"
     CDS             6163..6447
                     /codon_start=1
                     /product="mobC"
                     /label=mobC
                     /note="mobilization gene; derived from pRL1383a in INSD 
                     accession AF403426, a derivative of RSF1010 INSD accession 
                     M28829"
                     /protein_id="AII99703.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRERAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     rep_origin      6474..6868
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(7035..7078)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      complement(7044..7072)
                     /note="TrrnC; rrnC terminator from Lorist6 INSD accession 
                     X98450; Lorist6"
                     /regulatory_class="terminator"
     misc_feature    7111..7116
                     /label=NheI
                     /note="NheI"
     misc_feature    7117..7137
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    7135..7140
                     /label=ZraI
                     /note="ZraI"