pCVD023 vector (V001558)

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LOCUS       40924_49127        5124 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD023, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5124)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5124)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..536
                     /note="S7942NS2-RA; right arm of the homologous
                     recombination site NS2 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U44761"
     misc_feature    445..450
                     /label=SacI
                     /note="SacI"
     rep_origin      573..1161
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1347..1487)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(1567..1656)
                     /note="tet-3UTR; 3' UTR of the tetracyclin resistance ORF;
                     from pACYC184 INSD accession X06403"
     CDS             complement(1660..2847)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(2895..2923)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_recomb     2972..3913
                     /note="S7942NS2-LA; left arm of the homologous
                     recombination site NS2 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U44761"
     misc_feature    3914..3919
                     /label=NheI
                     /note="NheI"
     misc_feature    3920..3940
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    3938..3943
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(4067..4924)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4925..5029)
                     /label=AmpR promoter
     misc_feature    5055..5060
                     /label=ZraI
                     /note="ZraI"