Ubi-stop-mCD8GFP vector (V001582)

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LOCUS       40924_49002       14482 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector Ubi-stop-mCD8GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
  TITLE     Cookie monster binding to the repressed chromosomal domains is 
            needed for selective activation of testis-specific genes in 
            Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14482)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Genomics lab, Institute of molecular and 
            cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE   3  (bases 1 to 14482)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14482)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2013) Genomics lab, Institute of molecular and cellular 
            biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        224..2041
                     /label=Polyubiquitin
                     /note="Promoter of polyubiqutin gene of Drosophila
                     melanogaster"
     misc_recomb     2094..2127
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    2094..2127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    2154..3026
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(4210..4243)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4312..4977
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIXTLICYHSR
                     "
     CDS             4984..5697
                     /codon_start=1
                     /label=GFP
                     /note="green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQEXTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          5784..5849
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5979..5999
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    6271..6405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            6757..10302
                     /gene="mini-white"
                     /label=mini-white
     protein_bind    10481..10550
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(10904..11489)
                     /label=P element 5' end
     rep_origin      complement(11724..12312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12486..13343)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13344..13448)
                     /label=AmpR promoter
     misc_feature    complement(14250..14482)
                     /label=P element 3' end
                     /note="P element 3' end"