SW2 vector (V001592)

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LOCUS       40924_48872        6451 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector SW2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Yang XW, Jian HH, Wang FP.
  TITLE     A novel low temperature-inducible gene expression vector pSW2 based 
            on a filamentous phage of the deep-sea bacterium Shewanella 
            piezotolerans WP3
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26048946
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Yang X-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2015) School of Life Sciences and Biotechnology, 
            Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 
            Shanghai 200240, China
REFERENCE   3  (bases 1 to 6451)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6451)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAR-2015) School of Life Sciences and Biotechnology, Shanghai 
            Jiao Tong University, 800 Dongchuan Road, Shanghai, Shanghai 200240,
            China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             622..2247
                     /codon_start=1
                     /product="FpsA"
                     /label=FpsA
                     /note="putative replication protein"
                     /protein_id="AKM49063.1"
                     /translation="MAEGDIKDLAKEKAQQLTKQLATVDDDAERFRLAERSLSFLGLSF
                     DAATVKRKQEAGELFSMVNRAADEYWLRRQLRRRFAQSVEHVARDLALVQKHKQVYCSD
                     FSVNRYRARTEDNRAALENTVAYDEDDASNVFSLAELSDKSVSNVDNRNAEMFIRLKGF
                     EAVSQSLGHSAIFTTVTAPSRFHSVANRVTNKKWLAAGKPTPVQANDYLVEVWTALRKS
                     LDKKKIKIYGMRVVEPHHDGTPHHHQLIFGLPTDLAFVKKELKRLALLDSPNEKGAKEH
                     RFKCEDIDPNYTKKDGSKGSAVGYIAKYISKSTDGKHIEKDLTTDADAAKSAERIVTWA
                     RIENIRQFQFFGGPSVTVWREMRRLKDEIQEGDAVFNNLEGTEHYLLEKVRKSADEGDW
                     AAFCIAMGGVFVKRTDQTVRTHYAVPAVVEKLFESHEYATTRFGDKANARINGILFQGV
                     FKATRFKNWVVMNKEAYIRGQKKVMSGVVDIFDVLEREKEYERMAEQQYQEYENHMFEC
                     EEMQALLFEASAWRGSEATDVMTH"
     CDS             2309..2623
                     /codon_start=1
                     /product="FpsB"
                     /label=FpsB
                     /note="ssDNA binding protein"
                     /protein_id="AKM49064.1"
                     /translation="MKLEGLILDKSDIQTKQKTNFNTGEVNDVGLIKLITTYPTQTVDV
                     HVSVELMKDPKTLQVLNGVVGTRTQLSVEYKEMSFANSDGKHVAINGFHLFELPHVKAS
                     "
     CDS             2761..2946
                     /codon_start=1
                     /product="FpsC"
                     /label=FpsC
                     /note="putative capsid protein"
                     /protein_id="AKM49065.1"
                     /translation="MSRNSEYEARQKSKGLRKVTLWVPCHCENEFRLMAQSCCEDKDLI
                     PFMLRSLSTGQMRKGS"
     CDS             3040..3279
                     /codon_start=1
                     /product="FpsD"
                     /label=FpsD
                     /note="minor capsid protein"
                     /protein_id="AKM49066.1"
                     /translation="MSQCVAFTQEGYLVQSTQSVDDCTGYILVSPTEYNLAIQSIEIDP
                     ADVLQVFGLVFGWVVFLSFISYKVKIAKVLINRV"
     CDS             complement(3486..4142)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4143..4245)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(4315..4903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            5038..5145
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(6101..6451)
                     /codon_start=1
                     /product="FpsR"
                     /label=FpsR
                     /note="putative replication regulator"
                     /protein_id="AKM49068.1"
                     /translation="MTIGENIKLKRKSLGMTRSELIDRSGVSTAQLSRMERGEQNNPNL
                     ETLVAISTALNTSIDEIVFGEQSTSSIYLSKTIDTLPIDKKTFIKEVIKMAVMISSSEN
                     LENGLKEKKKQG"