SV40p TV vector (V001593)

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LOCUS       40924_48867        6588 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector SV40p TV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Konishi Y, Karnan S, Takahashi M, Ota A, Damdindorj L, Hosokawa Y, 
            Konishi H.
  TITLE     A system for the measurement of gene targeting efficiency in human 
            cell lines using an antibiotic resistance-GFP fusion gene
  JOURNAL   BioTechniques 53 (3), 141-152 (2012)
  PUBMED    22963476
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Konishi H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2012) Aichi Medical University School of Medicine,
            1-1 Yazako-Karimta, Nagakute, Aichi 480-1195, Japan
REFERENCE   3  (bases 1 to 6588)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6588)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2012"; volume: "53"; issue: "3"; pages: 
            "141-152"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JAN-2012) Aichi Medical University School of Medicine, 1-1 
            Yazako-Karimta, Nagakute, Aichi 480-1195, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     CDS             180..896
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    912..1136
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1180..1213
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1366..1723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1774..2565
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    2632..2680
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    2743..2776
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(2743..2776)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(2803..2819)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2822..3840
                     /note="partial SV40 largeT; an internal portion of SV40
                     largeT exon 2"
     repeat_region   3851..3991
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4066..4521
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4803..4907
                     /label=AmpR promoter
     CDS             4908..5765
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5939..6527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"