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pENTR-GFP-MDC1 (770-7) vector (V001666)

Basic Vector Information

Vector Name:
pENTR-GFP-MDC1 (770-7)
Antibiotic Resistance:
Kanamycin
Length:
9539 bp
Type:
Entry vector
Replication origin:
ori
Copy Number:
High Copy
Cloning Method:
Restriction Enzyme
5' Primer:
ENTRforw
3' Primer:
ENTRrev

pENTR-GFP-MDC1 (770-7) vector Vector Map

pENTR-GFP-MDC1 (770-7)9539 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088009200rrnB T1 terminatorrrnB T2 terminatorpENTR-FattL1EGFPMediator of DNA damage checkpoint protein 1attL2KanRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pENTR-GFP-MDC1 (770-7) vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V001666                 9539 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001666
VERSION     V001666
KEYWORDS    pENTR-GFP-MDC1 (770-7).
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9539)
  TITLE     New vectors Aug. 2010
REFERENCE   2  (bases 1 to 9539)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9539)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     316..339
                     /label="pENTR-F"
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /label="attL1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             504..1220
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1287..7553
                     /gene="MDC1"
                     /label="Mediator of DNA damage checkpoint protein 1"
                     /note="Mediator of DNA damage checkpoint protein 1 from
                     Homo sapiens. Accession#: Q14676"
     protein_bind    complement(7768..7867)
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7990..8796
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      8889..9477
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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