Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V001748 | pMo130-TelR | In stock, instant shipping |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pMo130-TelR
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9409 bp
- Type:
- Suicide vector
- Replication origin:
- ori
- Selection Marker:
- Tellurite
- Promoter:
- tac
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- 5'-CGGGTACCGAGCTCGAATTA-3'
- 3' Primer:
- 5'-CCGGGTACCGAGCTCGAATT-3'
- Growth Strain(s):
- DH10B
- Growth Temperature:
- 37℃
pMo130-TelR vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pMo130-TelR vector Sequence
LOCUS V001748 9409 bp DNA circular SYN 13-MAY-2021
DEFINITION Exported.
ACCESSION V001748
VERSION V001748
KEYWORDS pMo130-TelR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9409)
AUTHORS Amin IM, Richmond GE, Sen P, Koh TH, Piddock LJ, Chua KL
TITLE A method for generating marker-less gene deletions in
multidrug-resistant Acinetobacter baumannii.
JOURNAL BMC Microbiol. 2013 Jul 13;13:158. doi: 10.1186/1471-2180-13-158.
PUBMED 23848834
REFERENCE 2 (bases 1 to 9409)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 9409)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BMC
Microbiol."; date: "2013-07-13"; pages: "
10.1186/1471-2180-13-158"
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9409
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(972..1003)
/label="tonB terminator"
/note="bidirectional E. coli tonB-P14 transcription
terminator"
protein_bind complement(1067..1114)
/label="FRT"
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
rep_origin complement(1293..1881)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
oriT complement(2181..2290)
/direction=LEFT
/label="oriT"
/note="incP origin of transfer"
protein_bind complement(3501..3522)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
terminator 3540..3583
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(3600..4952)
/label="SacB"
/note="secreted levansucrase that renders bacterial growth
sensitive to sucrose"
protein_bind complement(5078..5094)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS complement(5156..5947)
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
promoter complement(5948..6051)
/label="AmpR promoter"
primer_bind 6119..6137
/label="pBRforEco"
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6175..6197)
/label="pGEX 3'"
/note="pGEX vectors, reverse primer"
CDS complement(6288..7208)
/gene="xylE"
/label="Metapyrocatechase"
/note="Metapyrocatechase from Pseudomonas putida.
Accession#: P06622"
promoter complement(7240..7268)
/label="tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind complement(7319..7366)
/label="FRT"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
RBS 7458..7480
/label="RBS"
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"