pZNR0926 vector (V001831)

Download GeneBank File(.gb)

LOCUS       V001831                 3156 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001831
VERSION     V001831
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.
  TITLE     An enzyme-coupled biosensor enables (S)-reticuline production in
            yeast from glucose
  JOURNAL   Nat. Chem. Biol. 11 (7), 465-471 (2015)
   PUBMED   25984720
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber
            JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Department of Bioengineering, University of
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA
            94704, USA
REFERENCE   3  (bases 1 to 3156)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3156)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-APR-2015) Department of Bioengineering, University of
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA
            94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..8
                     /label="BsaI recognition site"
                     /note="BsaI recognition site"
     CDS             11..1501
                     /gene="CYP76AD1"
                     /label="Cytochrome P450 76AD1"
                     /note="Cytochrome P450 76AD1 from Beta vulgaris.
                     Accession#: I3PFJ5"
     misc_feature    complement(1504..1507)
                     /label="BsaI recognition site"
                     /note="BsaI recognition site"
     misc_feature    complement(1509..1514)
                     /label="BsaI recognition site"
                     /note="BsaI recognition site"
     terminator      complement(1521..1555)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1629..2285)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2286..2389)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2467..3055
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"