pZIKV-ICD vector (V001846)

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LOCUS       40924_48398       14426 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Zika virus vector pZIKV-ICD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Tsetsarkin KA, Kenney H, Chen R, Liu G, Manukyan H, Whitehead SS, 
            Laassri M, Chumakov K, Pletnev AG.
  TITLE     A Full-Length Infectious cDNA Clone of Zika Virus from the 2015 
            Epidemic in Brazil as a Genetic Platform for Studies of Virus-Host 
            Interactions and Vaccine Development
  JOURNAL   MBio 7 (4), e01114-16 (2016)
  PUBMED    27555311
REFERENCE   2  (bases 1 to 14426)
  AUTHORS   Tsetsarkin KA, Pletnev AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2016) Neurotropic Flaviviruses Section; Laboratory
            of Infectious Diseases, National Institute of Allergy and Infectious
            Diseases, 3 North Dr Rm 3W10A, Bethesda, MD 20892-3203, USA
REFERENCE   3  (bases 1 to 14426)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14426)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio"; 
            date: "2016"; volume: "7"; issue: "4"; pages: "e01114-16"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2016) Neurotropic Flaviviruses Section; Laboratory of 
            Infectious Diseases, National Institute of Allergy and Infectious 
            Diseases, 3 North Dr Rm 3W10A, Bethesda, MD 20892-3203, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          9089..11073
                     /isolate="Paraiba_01/2015"
                     /mol_type="other DNA"
                     /db_xref="taxon:64320"
                     /organism="Zika virus"
     CDS             1874..1885
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     intron          2712..2844
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(8609..8620)
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
     intron          8956..9088
                     /note="derived from commercial plasmid pCI-neo"
     polyA_signal    11283..11364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    11399..11454
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(12181..12725)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(12893..13750)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(13751..13855)
                     /label=AmpR promoter
     enhancer        13904..14207
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14208..14411
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"