pZH3 vector (V001847)

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LOCUS       40924_48393        5832 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZH3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Hu JC.
  TITLE     Repressor fusions as a tool to study protein-protein interactions
  JOURNAL   Structure 3 (5), 431-433 (1995)
  PUBMED    7663940
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Zhu H, Celinski SA, Scholtz JM, Hu JC.
  TITLE     The contribution of buried polar groups to the conformational 
            stability of the GCN4 coiled coil
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5832)
  AUTHORS   Zhu H, Celinski SA, Scholtz JM, Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   4  (bases 1 to 5832)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5832)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure";
            date: "1995"; volume: "3"; issue: "5"; pages: "431-433"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (13-JUL-1999) Biochemistry "
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..40
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     terminator      complement(408..455)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(525..590)
                     /codon_start=1
                     /label=GCN4_v1
                     /note="GCN4 peptide that allows binding to an scFv-GFP
                     fusion protein (Tanenbaum et al., 2014)"
                     /translation="LLSKNYHLENEVARLKKLVGER"
     CDS             complement(2497..2535)
                     /codon_start=1
                     /product="expressed peptide start"
                     /label=expressed peptide start
                     /protein_id="AAD50824.1"
                     /translation="MSTDRSRRFTTS"
     RBS             complement(2543..2565)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(2597..2615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3386..3574
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    3679..3821
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4007..4595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4769..5626)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5627..5731)
                     /label=AmpR promoter