pZH2Bik vector (V001848)

LOCUS       40924_48388       12329 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pZH2Bik DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Kuroda M, Kimizu M, Mikami C.
  TITLE     A simple set of plasmids for the production of transgenic plants
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (11), 2348-2351 (2010)
  PUBMED    21071849
REFERENCE   2  (bases 1 to 12329)
  AUTHORS   Kuroda M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2010) Contact:Masaharu Kuroda National 
            Agricultural Research Center, Rice Physiology Research Team; Inada 
            1-2-1, Joetsu, Niigata 943-0193, Japan
REFERENCE   3  (bases 1 to 12329)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "11"; 
            pages: "2348-2351"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAR-2010) Contact:Masaharu Kuroda National Agricultural Research
            Center, Rice Physiology Research Team; Inada 1-2-1, Joetsu, Niigata 
            943-0193, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Mar 4, 2011 this sequence version replaced AB551245.1.
FEATURES             Location/Qualifiers
     source          1..12329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1813
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2245..3315
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5083..5871)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    6399..6423
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6638..6654
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7162..7507
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             7523..8545
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      8580..8829
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      8843..10590
                     /gene="Nos"
                     /note="derived from rice, ubi1 gene promoter with first
                     intron"
                     /regulatory_class="promoter"
     misc_feature    10615..11598
                     /gene="Nos"
                     /note="derived from rice, third intron of aspartic protease
                     coding gene"
     terminator      11636..11888
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11920..11936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11944..11960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11968..11998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12013..12034)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    12192..12216
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"