pZeoSv2-Furin vector (V001852)

Download GeneBank File(.gb)

LOCUS       V001852                 5868 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001852
VERSION     V001852
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Liu J, Jonebring A, Hagstrom J, Nystrom AC, Lovgren A.
  TITLE     Improved Expression of Recombinant Human Factor IX by Co-expression
            of GGCX, VKOR and Furin
  JOURNAL   Protein J. 33 (2), 174-183 (2014)
   PUBMED   24567122
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Liu J, Jonebring A, Hagstrom J, Nystrom A-C., Lovgren A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2013) Astrazeneca R
REFERENCE   3  (bases 1 to 5868)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5868)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein
            J."; date: "2014"; volume: "33"; issue: "2"; pages: "174-183"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-NOV-2013) Astrazeneca R"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..351
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        401..419
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             456..2837
                     /gene="FURIN"
                     /label="Furin"
                     /note="Furin from Homo sapiens. Accession#: P09958"
     polyA_signal    2919..3143
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3226..3814)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      4169..4624
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     enhancer        4660..4963
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4964..5167
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        5285..5332
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5351..5722
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5739..5868
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"