pZC320 vector (V001857)

Download GeneBank File(.gb)

LOCUS       40924_48333        7528 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZC320, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Shi J, Biek DP.
  TITLE     A versatile low-copy-number cloning vector derived from plasmid F
  JOURNAL   Gene 164 (1), 55-58 (1995)
  PUBMED    7590321
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Mori H, Kondo A, Ohshima A, Ogura T, Hiraga S.
  TITLE     Structure and function of the F plasmid genes essential for 
            partitioning
  JOURNAL   J. Mol. Biol. 192 (1), 1-15 (1986)
  PUBMED    3029390
REFERENCE   3  (bases 1 to 7528)
  AUTHORS   Prentki P, Binda A, Epstein A.
  TITLE     Plasmid vectors for selecting IS1-promoted deletions in cloned DNA: 
            sequence analysis of the omega interposon
  JOURNAL   Gene 103 (1), 17-23 (1991)
  PUBMED    1652541
REFERENCE   4  (bases 1 to 7528)
  AUTHORS   Biek DP.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   5  (bases 1 to 7528)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 7528)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1995"; volume: "164"; issue: "1"; pages: "55-58"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J. Mol. 
            Biol."; date: "1986"; volume: "192"; issue: "1"; pages: "1-15"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Gene"; 
            date: "1991"; volume: "103"; issue: "1"; pages: "17-23"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-1995) Donald P. Biek, Department of Microbiology "
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      74..293
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             384..1136
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    1142..1392
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             1718..2890
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             2893..3861
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    3937..4410
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     regulatory      4785..4922
                     /standard_name="omega terminator"
                     /label=omega terminator
                     /note="contains the T4 gene 32 terminator and a portion of
                     the polylinker from the omega interposon"
                     /regulatory_class="terminator"
     CDS             complement(5252..6109)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(6122..6181)
                     /codon_start=1
                     /gene="aadA'"
                     /product="aminoglycoside 3'-acetyltransferase"
                     /number=1
                     /label=aadA'
                     /note="encodes the first 18 amino acids of AadA and one
                     from the vector before stopping 12 bp from the beginning of
                     bla coding region"
                     /protein_id="AAC53639.1"
                     /translation="MREAVIAEVSTQLSEVVGD"
     gene            complement(6122..6181)
                     /gene="aadA'"
                     /label=aadA'
     primer_bind     6918..6934
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7027..7045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7067..7083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7091..7107)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7115..7145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7160..7181)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      complement(7399..7528)
                     /standard_name="omega terminator"
                     /label=omega terminator
                     /note="contains the T4 gene 32 terminator and portion of
                     the polylinker from the omega interposon"
                     /regulatory_class="terminator"