pZ1918 with lacZ cassette vector (Cat. No.: V001859)

LOCUS       beta-lactamase;        5932 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZ1918 with lacZ cassette, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    beta-lactamase; beta-galactosidase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Schweizer HP.
  TITLE     Two plasmids, X1918 and Z1918, for easy recovery of the xylE and 
            lacZ reporter genes
  JOURNAL   Gene 134 (1), 89-91 (1993)
  PUBMED    8244036
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Su TZ, Schweizer H, Oxender DL.
  TITLE     A novel phosphate-regulated expression vector in Escherichia coli
  JOURNAL   Gene 90 (1), 129-133 (1990)
  PUBMED    2379833
REFERENCE   3  (bases 458 to 532)
  AUTHORS   Simons RW, Houman F, Kleckner N.
  TITLE     Improved single and multicopy lac-based cloning vectors for protein 
            and operon fusions
  JOURNAL   Gene 53 (1), 85-96 (1987)
  PUBMED    3596251
REFERENCE   4  (bases 3619 to 3647)
  AUTHORS   Casadaban MJ, Martinez-Arias A, Shapira SK, Chou J.
  TITLE     Beta-galactosidase gene fusions for analyzing gene expression in 
            escherichia coli and yeast
  JOURNAL   Meth. Enzymol. 100, 293-308 (1983)
  PUBMED    6312261
REFERENCE   5  (bases 1 to 5932)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-1993) Herbert P. Schweizer, Microbiology and 
            Infectious Diseases, University of Calgary, 3330 Hospital Drive 
            N.W., Calgary, Alberta, T2N 4N1, Canada
REFERENCE   6  (bases 1 to 5932)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1993"; volume: "134"; issue: "1"; pages: "89-91"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Gene"; 
            date: "1990"; volume: "90"; issue: "1"; pages: "129-133"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Gene"; 
            date: "1987"; volume: "53"; issue: "1"; pages: "85-96"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Meth. 
            Enzymol. 100, 293-308 (1983)"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (03-DEC-1993) Herbert P. Schweizer, Microbiology and Infectious 
            Diseases, University of Calgary, 3330 Hospital Drive N.W., Calgary, 
            Alberta, T2N 4N1, Canada"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(399..454)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    458..532
                     /label=Escherichia coli trp operon DNA
                     /note="Escherichia coli trp operon DNA"
                     /citation=[3]
     regulatory      539..542
                     /gene="lacZ"
                     /regulatory_class="ribosome_binding_site"
     CDS             550..3621
                     /label=lacZ
                     /note="beta-galactosidase"
     misc_feature    3619..3647
                     /label=junction between lac operon DNA and polylinker
                     /note="junction between lac operon DNA and polylinker"
                     /citation=[4]
     misc_feature    3643..3698
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3711..3727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3735..3751)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3759..3789)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3804..3825)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4113..4701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4875..5732)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5733..5837)
                     /label=AmpR promoter