pYZ81N vector (V001860)

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LOCUS       40924_48318        9208 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYZ81N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Zhao Y, Elder RT, Chen M, Cao J.
  TITLE     Fission yeast expression vectors adapted for positive identification
            of gene insertion and green fluorescent protein fusion
  JOURNAL   BioTechniques 25 (3), 438-440 (1998)
  PUBMED    9762442
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Zhao Y, Chen M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-1998) Departments of Pediatrics and 
            Microbiology-Immunology, Northwestern University Medical School, 
            2430 N Halsted Street, #218, Chicago, IL 60614, USA
REFERENCE   3  (bases 1 to 9208)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "25"; issue: "3"; pages: 
            "438-440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-1998) Departments of Pediatrics and Microbiology-Immunology,
            Northwestern University Medical School, 2430 N Halsted Street, #218,
            Chicago, IL 60614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1205
                     /label=nmt1 P41 promoter
                     /note="mutant nmt1 promoter from Schizosaccharomyces pombe,
                     conferring medium strength thiamine-repressible expression"
     primer_bind     1350..1366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1367..1423
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1436..1452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1460..1476)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1484..1514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1529..1550)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      1649..2656
                     /label=from Schizosaccharomyces pombe nmt1
                     /note="from Schizosaccharomyces pombe nmt1"
                     /regulatory_class="terminator"
     rep_origin      complement(2729..3512)
                     /direction=LEFT
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(3862..3878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4091..4546
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4828..4932
                     /label=AmpR promoter
     CDS             4933..5790
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5964..6552
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6840..6861
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6876..6906
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6914..6930
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6938..6954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7458..8549)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(8562..8966)
                     /label=LEU2 promoter