Basic Vector Information
- Vector Name:
- pYZ41N
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9211 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Zhao Y, Elder RT, Chen M, Cao J.
- Promoter:
- nmt1
pYZ41N vector Map
pYZ41N vector Sequence
LOCUS 40924_48313 9211 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pYZ41N, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9211) AUTHORS Zhao Y, Elder RT, Chen M, Cao J. TITLE Fission yeast expression vectors adapted for positive identification of gene insertion and green fluorescent protein fusion JOURNAL BioTechniques 25 (3), 438-440 (1998) PUBMED 9762442 REFERENCE 2 (bases 1 to 9211) AUTHORS Zhao Y, Chen M. TITLE Direct Submission JOURNAL Submitted (06-MAY-1998) Departments of Pediatrics and Microbiology-Immunology, Northwestern University Medical School, 2430 N Halsted Street, #218, Chicago, IL 60614, USA REFERENCE 3 (bases 1 to 9211) TITLE Direct Submission REFERENCE 4 (bases 1 to 9211) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BioTechniques"; date: "1998"; volume: "25"; issue: "3"; pages: "438-440" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (06-MAY-1998) Departments of Pediatrics and Microbiology-Immunology, Northwestern University Medical School, 2430 N Halsted Street, #218, Chicago, IL 60614, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..9211 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 48..1208 /label=nmt1 promoter /note="wild-type nmt1 promoter from Schizosaccharomyces pombe, conferring strong thiamine-repressible expression" primer_bind 1353..1369 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 1370..1426 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(1439..1455) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1463..1479) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1487..1517) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1532..1553) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." regulatory 1652..2659 /label=from Schizosaccharomyces pombe nmt1 /note="from Schizosaccharomyces pombe nmt1" /regulatory_class="terminator" rep_origin complement(2732..3515) /direction=LEFT /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" primer_bind complement(3865..3881) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 4094..4549 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4831..4935 /label=AmpR promoter CDS 4936..5793 /label=AmpR /note="beta-lactamase" rep_origin 5967..6555 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 6843..6864 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 6879..6909 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6917..6933 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6941..6957 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS complement(7461..8552) /label=LEU2 /note="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" promoter complement(8565..8969) /label=LEU2 promoter
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