pYZ3N-GFP vector (V001862)

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LOCUS       40924_48308        9956 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYZ3N-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Zhao Y, Elder RT, Chen M.
  TITLE     Fission yeast expression vector with GFP tag and positive selection 
            for insert
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Zhao Y, Chen M, Elder RT, Cao J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-1998) Departments of Pediatrics and 
            Microbiology-Immunology, Northwestern University Medical School, 
            2430 N Halsted Street, #218, Chicago, IL 60614, USA
REFERENCE   3  (bases 1 to 9956)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-FEB-1998) Departments of Pediatrics and Microbiology-Immunology,
            Northwestern University Medical School, 2430 N Halsted Street, #218,
            Chicago, IL 60614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1210
                     /label=nmt1 promoter
                     /note="wild-type nmt1 promoter from Schizosaccharomyces
                     pombe, conferring strong thiamine-repressible expression"
     CDS             1225..1938
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     primer_bind     2094..2110
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2111..2167
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2180..2196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2204..2220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2228..2258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2273..2294)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      2394..3403
                     /label=from Schizosaccharomyces pombe nmt1
                     /note="from Schizosaccharomyces pombe nmt1"
                     /regulatory_class="terminator"
     rep_origin      complement(3477..4260)
                     /direction=LEFT
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(4610..4626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4839..5294
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5576..5680
                     /label=AmpR promoter
     CDS             5681..6538
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6712..7300
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7588..7609
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7624..7654
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7662..7678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7686..7702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(8206..9297)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(9310..9714)
                     /label=LEU2 promoter