pYUB28b vector (V001865)

LOCUS       V001865                 4921 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001865
VERSION     V001865
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN.
  TITLE     Metabolic Engineering of Cofactor F(420) Production in Mycobacterium
            smegmatis
  JOURNAL   PLoS ONE 5 (12), E15803 (2010)
   PUBMED   21209917
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Bashiri G, Baker EN.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2010) School of Biological Sciences, The
            University of Auckland, 3a Symonds St., Auckland, Auckland 1010, New
            Zealand
REFERENCE   3  (bases 1 to 4921)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2010"; volume: "5"; issue: "12"; pages: "E15803"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-SEP-2010) School of Biological Sciences, The University of
            Auckland, 3a Symonds St., Auckland, Auckland 1010, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..79
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             160..177
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             187..204
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             208..240
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             289..306
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      373..420
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      457..885
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             join(3928..4921,1..2)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"