pYT923hyg vector (V001866)

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LOCUS       V001866                11784 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001866
VERSION     V001866
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Nomoto M, Ogawa M, Fukuda K, Miyamoto H, Taniguchi H.
  TITLE     A host-vector system for molecular study of the intracellular growth
            of Mycobacterium tuberculosis in phagocytic cells
  JOURNAL   Microbiol. Immunol. 53 (10), 550-558 (2009)
   PUBMED   19780968
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Fukuda K, Taniguchi H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2009) Contact:Kazumasa Fukuda University of
            Occupational and Environmental Health, JAPAN, Department of
            Microbiology, School of Medicine; 1-1 ISEIGAOKA,YAHATANISHI-KU,
            KITAKYUSHU, FUKUOKA 807-8555, Japan
REFERENCE   3  (bases 1 to 11784)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11784)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol.
            Immunol."; date: "2009"; volume: "53"; issue: "10"; pages: "550-558"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JUL-2009) Contact:Kazumasa Fukuda University of Occupational and
            Environmental Health, JAPAN, Department of Microbiology, School of
            Medicine; 1-1 ISEIGAOKA,YAHATANISHI-KU, KITAKYUSHU, FUKUOKA
            807-8555, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..2505
                     /label="replication region in BCG"
                     /note="replication region in BCG"
     CDS             complement(4693..5505)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(6100..6645)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6813..7670)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7671..7775)
                     /label="AmpR promoter"
     CDS             complement(8596..9591)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     primer_bind     complement(9797..9813)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    9821..9837
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9845..9875)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9890..9911)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    10381..10521
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10707..11295)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"