pYT3 vector (V001867)

LOCUS       40924_48278        7596 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pYT3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Tan X, Waterham HR, Veenhuis M, Cregg JM.
  TITLE     The Hansenula polymorpha PER8 gene encodes a novel peroxisomal 
            integral membrane protein involved in proliferation
  JOURNAL   J. Cell Biol. 128 (3), 307-319 (1995)
  PUBMED    7844145
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Baerends RJS., van Dijk R, Cregg JM, Veenhuis M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2001) Eukaryotic Microbiology, Groningen 
            Biomolecular Sciences and Biotechnology Institute, Kerklaan 30, 
            Haren, Gn 9751 NN, the Netherlands
REFERENCE   3  (bases 1 to 7596)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Cell 
            Biol."; date: "1995"; volume: "128"; issue: "3"; pages: "307-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-FEB-2001) Eukaryotic Microbiology, Groningen Biomolecular 
            Sciences and Biotechnology Institute, Kerklaan 30, Haren, Gn 9751 
            NN, the Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..2724
                     /note="derived from Hansenula polymorpha; confers
                     autonomous replication activity (HARS) in Hansenula 
                     polymorpha"
     RBS             1139..1147
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(3218..4309)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(4310..4717)
                     /label=LEU2 promoter
     primer_bind     complement(4989..5005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5013..5029)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5037..5067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5082..5103)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5391..5979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6153..7010)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7011..7115)
                     /label=AmpR promoter