pYOGM081 vector (V001872)

LOCUS       40924_48228        8332 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYOGM081, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Labunskyy VM, Suzuki Y, Hanly TJ, Murao A, Roth FP, Gladyshev VN.
  TITLE     The insertion Green Monster (iGM) method for expression of multiple 
            exogenous genes in yeast
  JOURNAL   G3 (Bethesda) 4 (7), 1183-1191 (2014)
  PUBMED    24776987
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Suzuki Y, Roth FP.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2014) Synthetic Biology and Bioenergy, J. Craig 
            Venter Institute, 4120 Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 8332)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)"; date: "2014"; volume: "4"; issue: "7"; pages: 
            "1183-1191"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JAN-2014) Synthetic Biology and Bioenergy, J. Craig Venter 
            Institute, 4120 Torrey Pines Road, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..70
                     /label=upstream linearization site
                     /note="upstream linearization site"
     misc_feature    33..152
                     /label=similar to the KanMX4 cassette
                     /note="similar to the KanMX4 cassette"
     promoter        167..383
                     /label=URA3 promoter
     CDS             384..1184
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     terminator      1295..1482
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     protein_bind    1499..1517
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1541..1559
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      1594..1746
                     /label=CYC1 TATA region
                     /note="CYC1 TATA region"
                     /regulatory_class="TATA_box"
     CDS             1755..2468
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     terminator      2493..2740
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        2756..3197
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     3212..3228
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3236..3360
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3385..3415
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3469..4125
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             4473..4775
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4819..4943)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4984..5073
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     terminator      5107..5304
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    5346..5404
                     /label=downstream linearization site
                     /note="downstream linearization site"
     primer_bind     complement(5424..5440)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5477..5495)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5516..5532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5540..5556)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5564..5594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5609..5630)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5918..6506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6680..7537)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7538..7642)
                     /label=AmpR promoter
     rep_origin      complement(7668..8123)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8265..8281
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8291..8309
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"