pYL4 vector (V001880)

LOCUS       V001880                 6275 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001880
VERSION     V001880
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Liu Y, Charpin-El Hamri G, Ye H, Fussenegger M.
  TITLE     A synthetic free fatty acid-regulated transgene switch in mammalian
            cells and mice
  JOURNAL   Nucleic Acids Res. (2018) In press
   PUBMED   30219861
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Liu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2018) Department of Biosystems Science and
            Engineering, ETH Zurich, Mattenstrasse 26, basel 4058, Switzerland
REFERENCE   3  (bases 1 to 6275)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6275)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2018) Department of Biosystems Science and Engineering, ETH
            Zurich, Mattenstrasse 26, basel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..919
                     /gene="FFAR1"
                     /label="Free fatty acid receptor 1"
                     /note="Free fatty acid receptor 1 from Homo sapiens.
                     Accession#: O14842"
     polyA_signal    960..1184
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1230..1658
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1672..2001
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2068..2859
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3036..3169
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3206..3222)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3230..3246)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3254..3284)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3299..3320)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3608..4193)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4367..5224)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5225..5329)
                     /label="AmpR promoter"
     enhancer        5595..5974
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5975..6178
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        6223..6241
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"