pYL2 vector (V001881)

Download GeneBank File(.gb)

LOCUS       40924_48148        5821 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYL2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Pomraning KR.
  TITLE     Yarrowia lipolytica functional genomics toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Pomraning KR.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2016) Environmental Molecular Science Lab, Pacific
            Northwest National Lab, 902 Battelle Blvd, Richland, WA 99352, USA
REFERENCE   3  (bases 1 to 5821)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-JAN-2016) Environmental Molecular Science Lab, Pacific Northwest
            National Lab, 902 Battelle Blvd, Richland, WA 99352, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     regulatory      382..1382
                     /gene="exp1"
                     /regulatory_class="promoter"
     gene            382..1382
                     /gene="exp1"
                     /label=exp1
     CDS             1431..2141
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     regulatory      2175..2579
                     /gene="tef"
                     /regulatory_class="promoter"
     gene            2175..2579
                     /gene="tef"
                     /label=tef
     CDS             2592..3614
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    3662..3906
                     /note="modified version of ARS68, contains centromere and
                     origin of replication sequences for Yarrowia lipolytica"
     rep_origin      complement(4137..4725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4902..5693)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5694..5798)
                     /label=AmpR promoter