pYL15 vector (V001882)

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LOCUS       V001882                 9748 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001882
VERSION     V001882
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Pomraning KR.
  TITLE     localization of lipid biogenesis in yarrowia lipolytica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Pomraning KR.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2016) Environmental Molecular Science Lab, Pacific
            Northwest National Lab, 902 Battelle Blvd, Richland, WA 99352, USA
REFERENCE   3  (bases 1 to 9748)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9748)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2016) Environmental Molecular Science Lab, Pacific Northwest
            National Lab, 902 Battelle Blvd, Richland, WA 99352, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             40..750
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     CDS             complement(1031..2245)
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Yarrowia
                     lipolytica (strain CLIB 122 / E 150). Accession#: P18120"
     misc_feature    3037..3281
                     /note="modified version of ARS68, contains centromere and
                     origin of replication sequences for Yarrowia lipolytica"
     rep_origin      complement(3512..4100)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4277..5068)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5069..5173)
                     /label="AmpR promoter"
     primer_bind     5489..5505
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5516..5534
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5539..5550
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             complement(8036..8047)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     regulatory      8995..9739
                     /gene="exp1"
                     /regulatory_class="promoter"
     gene            8995..9739
                     /gene="exp1"
                     /label="exp1"