pYL1 vector (V001883)

LOCUS       40924_48138        5229 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYL1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Liu Y, Charpin-El Hamri G, Ye H, Fussenegger M.
  TITLE     A synthetic free fatty acid-regulated transgene switch in mammalian 
            cells and mice
  JOURNAL   Nucleic Acids Res. (2018) In press
  PUBMED    30219861
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Liu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2018) Department of Biosystems Science and 
            Engineering, ETH Zurich, Mattenstrasse 26, basel 4058, Switzerland
REFERENCE   3  (bases 1 to 5229)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5229)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2018) Department of Biosystems Science and Engineering, ETH 
            Zurich, Mattenstrasse 26, basel 4058, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    14..21
                     /label=cAMP-response element (CRE)
                     /note="cAMP-response element (CRE)"
     misc_feature    40..44
                     /label=CRE
                     /note="CRE"
     misc_feature    50..54
                     /label=CRE
                     /note="CRE"
     misc_feature    72..79
                     /label=CRE
                     /note="CRE"
     misc_feature    109..118
                     /label=serum response factor binding element (SRE)
                     /note="serum response factor binding element (SRE)"
     misc_feature    132..141
                     /label=SRE
                     /note="SRE"
     misc_feature    155..164
                     /label=SRE
                     /note="SRE"
     protein_bind    189..218
                     /label=NFAT binding site
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    219..248
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     misc_feature    221..229
                     /label=NFAT
                     /note="NFAT"
     protein_bind    249..278
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     misc_feature    251..259
                     /label=NFAT
                     /note="NFAT"
     promoter        291..322
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             344..1852
                     /codon_start=1
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
                     /translation="PTMLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKL
                     QPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNV
                     DKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVG
                     VVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGG
                     RKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSV
                     THLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHE
                     SRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPG
                     KARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAV
                     FARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     polyA_signal    complement(1910..2031)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2696..3284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3458..4315)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4316..4420)
                     /label=AmpR promoter
     rep_origin      4447..4902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5033..5081
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5095..5186
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"