pYFPamy vector (V001890)

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LOCUS       V001890                 7420 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001890
VERSION     V001890
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Bisicchia P, Botella E, Devine KM.
  TITLE     Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP
            transcriptional fusions in single copy at the amyE and bglS loci in
            Bacillus subtilis
  JOURNAL   Plasmid 64 (3), 143-149 (2010)
   PUBMED   20600285
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Bisicchia P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace,
            Dublin 2, Ireland
REFERENCE   3  (bases 1 to 7420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2010"; volume: "64"; issue: "3"; pages: "143-149"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace, Dublin 2,
            Ireland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     115..651
                     /label="amyE front"
                     /note="amyE front"
     primer_bind     685..701
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        708..726
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     759..775
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(780..1496)
                     /label="EYFP"
                     /note="enhanced YFP"
     misc_feature    complement(1527..1562)
                     /label="LIC site"
                     /note="LIC site"
     CDS             1721..2368
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_recomb     2736..3772
                     /label="amyE back"
                     /note="amyE back"
     CDS             4972..5160
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5265..5405
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5591..6179)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6353..7210)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7211..7315)
                     /label="AmpR promoter"